Phosphotyrosine anticorps

N° du produit ABIN135058

Cet anticorps Souris Monoclonal détecte spécifiquement Phosphotyrosine dans ELISA.

Aperçu rapide pour Phosphotyrosine anticorps (ABIN135058)

Antigène: Phosphotyrosine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phosphotyrosine est non-conjugé

Application: ELISA

Clone: PY20

Détail du produit anti-Phosphotyrosine anticorps

Specificité: Tyrosine-phosphorylated proteins

Attributs du produit: Mouse Anti-Phosphotyrosine-UNLB

Purification: Purified

Immunogène: Phosphotyrosine conjugated to carrier proteins

Isotype: IgG2b

Information d'application

Indications d'application:

• Applications: ELISA - Quality tested , FLISA - Quality tested WB - Reported in literature , IP - Reported in literature , IHC-PS - Reported in literature , IHC-WM - Reported in literature , ICC - Reported in literature , FC - Reported in literature , Microarray - Reported in literature , Purification - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:1,000 - 1:4,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC conjugate 1:200 - 1:400 PE conjugate ≤ 1 g/mL

Volume d'échantillon: 1 mL

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Agent conservateur: Without preservative

Conseil sur la manipulation: Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C

Détails sur Phosphotyrosine

Antigène: Phosphotyrosine

Classe de substances: Amino Acid

Sujet: Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.