L-Selectin anticorps

N° du produit ABIN135249

Cet anticorps anti-L-Selectin est un anticorps Rat Monoclonal détectant L-Selectin dans FACS. Adapté pour Souris.

Aperçu rapide pour L-Selectin anticorps (ABIN135249)

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp L-Selectin est non-conjugé

Application: Flow Cytometry (FACS)

Clone: MEL-14

Détail du produit anti-L-Selectin anticorps

Specificité: Mouse CD62L, Mr 74 kDa on lymphocytes and Mr 95 kDa on neutrophils

Attributs du produit: Rat Anti-Mouse CD62L-UNLB

Purification: Purified

Immunogène: C3H/eb cloned mouse B cell lymphoma 38C-13

Isotype: IgG2a

Information d'application

Indications d'application:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Cyto - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PACBLU conjugate 0.3 g/106 cells PE, APC, SPRD, PE/CY7, APC/CY7, AF488, AF647, and AF700 0.1 g/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Commentaires:

In vivo and in vitro blocking of adhesion, Cytotoxicity studies

Volume d'échantillon: 1 mL

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Agent conservateur: Without preservative

Conseil sur la manipulation: Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C

Détails sur L-Selectin

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Autre désignation: CD62L

Classe de substances: Chemical

Sujet: CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.