H-2Kd/H-2Dd anticorps

N° du produit ABIN135416

Détail du produit

Antigène: H-2Kd/H-2Dd

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS)

Specificité: Mouse H-2Kd/H-2Dd

Attributs du produit: Mouse Anti-Mouse H-2Kd/H-2Dd-UNLB

Purification: Purified

Immunogène: BDF1 mouse splenocytes

Clone: 34-1-2S

Isotype: IgG2a

Information d'application

Indications d'application:

• Applications: FC - Quality tested , ICC - Reported in literature , EM - Reported in literature , IP - Reported in literature , CMCD - Reported in literature , Block - Reported in literature , Adhesion - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Commentaires:

Blocking Cell Adhesion, Complement-mediated cytotoxicity

Volume d'échantillon: 1 mL

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Agent conservateur: Without preservative

Conseil sur la manipulation: Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C

Détail du antigène

Antigène: H-2Kd/H-2Dd

Sujet: The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic chains noncovalently associated with the invariant 2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.