Cet anticorps anti-MHC Class II est un anticorps Souris Monoclonal détectant MHC Class II dans FACS. Adapté pour Poulet. Ce Primary Antibody a été cité dans 1 publication.
MHC2
Reactivité: Souris, Rat
FACS, IHC, IP
Hôte: Lapin
Chimeric
P7-7
unconjugated
Recombinant Antibody
Indications d'application
Applications: FC - Quality tested , IHC-FS - Reported in literature , EM - Reported in literature , IP - Reported in literature , Purification - Reported in literature
Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Volume d'échantillon
1 mL
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Agent conservateur
Without preservative
Conseil sur la manipulation
Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C
Stockage commentaire
Store at 2-8°C
Mucksová, Plachý, Staněk, Hejnar, Kalina, Benešová, Trefil: "Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines." dans: Veterinary research, Vol. 48, Issue 1, pp. 18, (2017) (PubMed).
Antigène
MHC Class II (MHC2)
(Major Histocompatibility Complex Class II Antigen (MHC2))
Autre désignation
MHC Class II
Sujet
The chicken major histocompatibility complex (MHC), or B complex, consists of several clusters of highly polymorphic genes. Like their mammalian counterparts, the avian MHC exerts genetic influence over a variety of important biological functions such as immune response, disease resistance, growth and development, aging, and reproduction. Chicken MHC Class II genes, also known as the B-L subregion, of the chicken MHC encode cell surface glycoproteins that are homologous to mammalian Class II antigens. B-L antigens structurally are similar to mammalian Class II molecules in that they are noncovalently bound dimmers of one 32-34 kDa heavy chain and one 27-30 kDa light chain. MHC Class II is primarily expressed on B cells and antigen presenting cells (APCs).