CARM1 anticorps (AA 226-230)

N° du produit ABIN1355824

Détail du produit anti-CARM1 anticorps

Antigène: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Épitope: AA 226-230

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CARM1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificité: The antibody detects endogenous levels of total CARM1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogène: Peptide sequence around AA 226-230 (V-K-S-N-N) derived from CARM1 Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détails sur CARM1

Antigène: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Autre désignation: CARM1 (CARM1 Produits)

Synonymes: anticorps NV18905, anticorps prmt4, anticorps PRMT4, anticorps Prmt4, anticorps si:dkey-204f11.63, anticorps zgc:100805, anticorps coactivator associated arginine methyltransferase 1, anticorps coactivator associated arginine methyltransferase 1 L homeolog, anticorps coactivator-associated arginine methyltransferase 1, anticorps histone-arginine methyltransferase CARM1, anticorps CARM1, anticorps carm1.L, anticorps Carm1, anticorps carm1, anticorps LOC100352221

Sujet: Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.

Poids moléculaire: 63 kDa

NCBI Accession: NP_954592

UniProt: Q86X55

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of fat Cell Differentiation