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Bu-1 anticorps (Biotin)

L’anticorps Souris Monoclonal anti-Bu-1 a été validé pour FACS. Il convient pour détecter Bu-1 dans des échantillons de Poulet. Il y a 1 publication disponible.
N° du produit ABIN135596

Aperçu rapide pour Bu-1 anticorps (Biotin) (ABIN135596)

Antigène

Bu-1

Reactivité

Poulet

Hôte

  • 9
Souris

Clonalité

  • 9
Monoclonal

Conjugué

  • 3
  • 3
  • 2
  • 1
Cet anticorp Bu-1 est conjugé à/à la Biotin

Application

  • 9
  • 6
  • 6
  • 5
Flow Cytometry (FACS)

Clone

AV20
  • Specificité

    Chicken Bu-1, Mr 70 kDa

    Attributs du produit

    Mouse Anti-Chicken Bu-1-BIOT

    Immunogène

    Chicken peripheral blood lymphocytes and splenocytes

    Isotype

    IgG1
  • Indications d'application

    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature
    • Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Volume d'échantillon

    1 mL

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg in 1.0 mL of PBS/Sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Stock

    4 °C

    Stockage commentaire

    Store at 2-8°C
  • Mucksová, Plachý, Staněk, Hejnar, Kalina, Benešová, Trefil: "Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines." dans: Veterinary research, Vol. 48, Issue 1, pp. 18, (2017) (PubMed).

  • Antigène

    Bu-1

    Sujet

    Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
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