RAPGEF3 anticorps (AA 301-400)

N° du produit ABIN1387844

L’anticorps Lapin Polyclonal anti-RAPGEF3 a été validé pour ELISA, ICC, IF (cc), IF (p), IHC (p) et IHC (fro). Il convient pour détecter RAPGEF3 dans des échantillons de Humain et Rat.

Aperçu rapide pour RAPGEF3 anticorps (AA 301-400) (ABIN1387844)

Antigène: RAPGEF3 (Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAPGEF3 est non-conjugé

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-RAPGEF3 anticorps

Épitope: AA 301-400

Réactivité croisée: Humain, Rat

Homologie: Mouse,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Epac1

Isotype: IgG

Information d'application

Indications d'application: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur RAPGEF3

Antigène: RAPGEF3 (Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))

Autre désignation: Epac1

Sujet:

Synonyms: bcm910, CAMP GEFI, cAMP regulated guanine nucleotide exchange factor I, CAMPGEFI, CGEF 1, CGEF1, EPA1, Epac 1, EPAC, EPAC1, Exchange factor directly activated by cAMP 1, Exchange protein directly activated by cAMP 1, MGC21410, RAP guanine nucleotide exchange factor, Rap guanine nucleotide exchange factor GEF 3, RAP guanine nucleotide exchange factor 3, Rap1 guanine nucleotide exchange factor directly activated by cAMP, RAPGEF3.

Background: The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The to Epac 1are affinity purified over immobilized antigen based chromatography.

ID gène: 1019

Pathways: cAMP Metabolic Process