LIN28A anticorps (AA 65-160)

N° du produit ABIN1387887

Cet anticorps anti-LIN28A est un anticorps Lapin Polyclonal détectant LIN28A dans WB, ELISA, IHC (p), IF (cc), IF (p) et IHC (fro). Adapté pour Humain et Rat.

Aperçu rapide pour LIN28A anticorps (AA 65-160) (ABIN1387887)

Antigène: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIN28A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-LIN28A anticorps

Épitope: AA 65-160

Réactivité croisée: Humain, Rat

Homologie: Mouse,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Lin-28B

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur LIN28A

Antigène: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Autre désignation: Lin-28

Sujet:

Synonyms: Lin28B, CSDD 2, CSDD2, FLJ16517, Lin 28 homolog B C. elegans, Lin 28.2, Lin-28B, LIN28B, LN28B_HUMAN, Protein lin-28 homolog B, Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1.

Background: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

ID gène: 389421, 79727

UniProt: Q6ZN17, Q9H9Z2

Pathways: Stem Cell Maintenance