Neuronatin (NNAT) (AA 31-81) anticorps

N° du produit ABIN1387892

L’anticorps Lapin Polyclonal anti- a été validé pour WB, ICC, IF (cc), IF (p), IHC (fro) et IHC (p). Il convient pour détecter dans des échantillons de Humain, Rat et Porc. Il y a 2+ publications disponibles.

Aperçu rapide pour Neuronatin (NNAT) (AA 31-81) anticorps (ABIN1387892)

Antigène: Neuronatin (NNAT)

Reactivité: Humain, Rat, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit

Épitope: AA 31-81

Réactivité croisée: Humain, Porc, Rat

Homologie: Mouse,Cow,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Neuronatin

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Référence

Gao, Chen, Wang, Nie, Zhang, Miao: "Oocyte aging-induced Neuronatin (NNAT) hypermethylation affects oocyte quality by impairing glucose transport in porcine." dans: Scientific reports, Vol. 6, pp. 36008, (2018) (PubMed).

Détail du antigène

Antigène: Neuronatin (NNAT)

Autre désignation: Neuronatin

Sujet:

Synonyms: Neuronatin, Nnat, NNAT_HUMAN, Peg 5, Peg5.

Background: The paternally imprinted Neuronatin gene (NNAT) is initially expressed in rhombomeres and the pituitary gland and is later expressed more widely, but much less abundantly, in the central and peripheral nervous systems. The human NNAT gene maps to chromosome 20q11.23 and contains an imprinting region associated with morphological abnormalities and early neonatal lethality. Specifically, hypermethylation of the NNAT gene occurs in both myeloid and lymphoid acute pediatric leukemias and may inhibit NNAT expression. The Neuronatin protein consists of two isoforms, alpha and beta, which are the products of alternative splicing. The alpha form of the Neuronatin gene is encoded by three exons, whereas the beta form is missing the second exon. Neuronatin mRNA expression is abundant in undifferentiated PC-12 cells. Treatment of these cells with nerve growth factor (NGF), which contributes to neuronal differentiation, downregulates Neuronatin mRNA expression. NNAT (-) 1.9 PC-12 cells exhibit an increase in nigericin, rotenone and valinomcin sensitivity, NNAT transfection restores wild-type PC-12 resistance. These results suggests a potential protective role for Neuronatin against toxic insult during development.

ID gène: 4826

UniProt: Q16517

Pathways: Positive Regulation of Peptide Hormone Secretion