LIN28A anticorps (AA 65-160) (HRP)

N° du produit ABIN1415223

Cet anticorps anti-LIN28A est un anticorps Lapin Polyclonal détectant LIN28A dans WB, ELISA, IHC (p) et IHC (fro). Adapté pour Humain et Rat.

Aperçu rapide pour LIN28A anticorps (AA 65-160) (HRP) (ABIN1415223)

Antigène: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIN28A est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-LIN28A anticorps (HRP)

Épitope: AA 65-160

Réactivité croisée: Humain, Rat

Homologie: Mouse,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Lin-28B

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur LIN28A

Antigène: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Autre désignation: Lin-28

Sujet:

Synonyms: Lin28B, CSDD 2, CSDD2, FLJ16517, Lin 28 homolog B C. elegans, Lin 28.2, Lin-28B, LIN28B, LN28B_HUMAN, Protein lin-28 homolog B, Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1.

Background: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

ID gène: 389421, 79727

UniProt: Q6ZN17, Q9H9Z2

Pathways: Stem Cell Maintenance