AMPK gamma 1/2/3 (AA 280-330) anticorps (HRP)

N° du produit ABIN1420630

Cet anticorps Lapin Polyclonal détecte spécifiquement dans WB et IHC (p). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour AMPK gamma 1/2/3 (AA 280-330) anticorps (HRP) (ABIN1420630)

Antigène: AMPK gamma 1/2/3

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit

Épitope: AA 280-330

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human PRKAG2/AMPK_2

Isotype: IgG

Information d'application

Indications d'application: WB 1:100-1000
IHC-P 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: AMPK gamma 1/2/3

Sujet:

Synonyms: 5'-AMP-activated protein kinase subunit gamma-2, AAKG, AMPK gamma2, AMPK gamma 2, AMPK subunit gamma 2, CMH6, H91620p, AMPKg, AMPK subunit gamma-1, AMPK gamma1, AMPKg3, AMPK subunit gamma-3, AMPK gamma3.

Background: AMPK is a heterotrimeric complex comprising a catalytic a subunit and regulatory b and g subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKa1 and AMPKa2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKb1 encodes a 271 amino acid protein and human AMPKb2 encodes a 272 amino acid protein. The human AMPKg1 gene encodes a 331 amino acid protein. Human AMPKg2 and AMPKg3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.

ID gène: 5571, 51422, 53632