GFP anticorps

N° du produit ABIN1439999

Cet anticorps Chèvre Polyclonal détecte spécifiquement GFP dans WB, IF, IHC (fro) et IHC (p). Il présente une réactivité avec des échantillons de Aequorea victoria. Il a été cité dans 18+ publications.

Aperçu rapide pour GFP anticorps (ABIN1439999)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GFP anticorps

Fonction: anti-GFP

Séquence: MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

Specificité: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.

Attributs du produit: Green Fluorescent Protein

Purification: Epitope affinity purified

Immunogène: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Information d'application

Indications d'application: WB:1:500-1:5,000, IF:1:50-1:1,000, IHC-P:1:50-1:1,000, IHC-F:1:50-1:1,000, IEM:1:50-1:1,000

Restrictions: For Research Use only

Stockage

Concentration: 2 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody solution should be gently mixed before use.

Stock: 4 °C,-20 °C

Stockage commentaire: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Références pour anticorps anti-GFP (ABIN1439999)

Panayiotou, Michael, Zaravinos, Demirag, Achilleos, Strati: "Human papillomavirus E7 binds Oct4 and regulates its activity in HPV-associated cervical cancers." dans: PLoS pathogens, Vol. 16, Issue 4, pp. e1008468, (2020) (PubMed).

Wu, Yi, Manca, Javaid, Lauer, Glowatzki: "Sound exposure dynamically induces dopamine synthesis in cholinergic LOC efferents for feedback to auditory nerve fibers." dans: eLife, Vol. 9, (2020) (PubMed).

Fukuda, Kaneshige, Kaji, Noguchi, Takemoto, Zhang, Tsujikawa, Kokubo, Uezumi, Maehara, Harada, Ohkawa, Fukada: "Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle." dans: eLife, Vol. 8, (2020) (PubMed).

Zhang, Uezumi, Kaji, Tsujikawa, Andersen, Jensen, Fukada: "Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration." dans: International journal of molecular sciences, Vol. 20, Issue 13, (2019) (PubMed).

Vyas, Wu, Jimenez, Glowatzki, Fuchs: "Characterization of transgenic mouse lines for labeling type I and type II afferent neurons in the cochlea." dans: Scientific reports, Vol. 9, Issue 1, pp. 5549, (2019) (PubMed).

Larson, Mironova, Vanderpool, Waisman, Rash, Agarwal, Bergles: "Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility." dans: eLife, Vol. 7, (2019) (PubMed).

Ramos-Nascimento, Kellen, Ferreira, Alenquer, Vale-Costa, Raposo, Delevoye, Amorim: "KIF13A mediates trafficking of influenza A virus ribonucleoproteins." dans: Journal of cell science, Vol. 130, Issue 23, pp. 4038-4050, (2018) (PubMed).

Wu, Vyas, Glowatzki, Fuchs: "Opposing expression gradients of calcitonin-related polypeptide alpha (Calca/Cgrpα) and tyrosine hydroxylase (Th) in type II afferent neurons of the mouse cochlea." dans: The Journal of comparative neurology, Vol. 526, Issue 3, pp. 425-438, (2018) (PubMed).

Festa, Chen, Berquez, Debaix, Tokonami, Prange, Hoek, Alessio, Raimondi, Nevo, Giles, Devuyst, Luciani: "Impaired autophagy bridges lysosomal storage disease and epithelial dysfunction in the kidney." dans: Nature communications, Vol. 9, Issue 1, pp. 161, (2018) (PubMed).

Mimura, Takagi, Clever, Imamoto: "ELYS regulates the localization of LBR by modulating its phosphorylation state." dans: Journal of cell science, Vol. 129, Issue 22, pp. 4200-4212, (2017) (PubMed).

Vale-Costa, Alenquer, Sousa, Kellen, Ramalho, Tranfield, Amorim: "Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors." dans: Journal of cell science, Vol. 129, Issue 8, pp. 1697-710, (2017) (PubMed).

Agarwal, Wu, Hughes, Fukaya, Tischfield, Langseth, Wirtz, Bergles: "Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes." dans: Neuron, Vol. 93, Issue 3, pp. 587-605.e7, (2017) (PubMed).

Vyas, Wu, Zimmerman, Fuchs, Glowatzki: "Tyrosine Hydroxylase Expression in Type II Cochlear Afferents in Mice." dans: Journal of the Association for Research in Otolaryngology : JARO, Vol. 18, Issue 1, pp. 139-151, (2017) (PubMed).

Martins-Marques, Anjo, Pereira, Manadas, Girão: "Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart." dans: Molecular & cellular proteomics : MCP, Vol. 14, Issue 11, pp. 3040-55, (2016) (PubMed).

Sargiannidou, Kim, Kyriakoudi, Eun, Kleopa: "A start codon CMT1X mutation associated with transient encephalomyelitis causes complete loss of Cx32." dans: Neurogenetics, Vol. 16, Issue 3, pp. 193-200, (2016) (PubMed).

Kourti, Ikonomou, Giakoumakis, Rapsomaniki, Landegren, Siniossoglou, Lygerou, Simos, Mylonis: "CK1δ restrains lipin-1 induction, lipid droplet formation and cell proliferation under hypoxia by reducing HIF-1α/ARNT complex formation." dans: Cellular signalling, Vol. 27, Issue 6, pp. 1129-40, (2015) (PubMed).

Paukert, Agarwal, Cha, Doze, Kang, Bergles: "Norepinephrine controls astroglial responsiveness to local circuit activity." dans: Neuron, Vol. 82, Issue 6, pp. 1263-70, (2014) (PubMed).

Pais, Milho, Almeida, Mota: "Identification of novel type III secretion chaperone-substrate complexes of Chlamydia trachomatis." dans: PLoS ONE, Vol. 8, Issue 2, pp. e56292, (2013) (PubMed).

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet:

Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.

Green fluorescent protein antibody.

Poids moléculaire: 26.9 kDa