SPARC anticorps

N° du produit ABIN1450173

Cet anticorps anti-SPARC est un anticorps Souris Monoclonal détectant SPARC dans WB, FACS, IF, IHC (p), IHC (fro), EIA et RIA. Adapté pour Humain, Rat et Boeuf (Vache).

Aperçu rapide pour SPARC anticorps (ABIN1450173)

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivité: Humain, Rat, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SPARC est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Clone: N50

Détail du produit anti-SPARC anticorps

Specificité: Recognizes Human and Bovine bone Osteonectin, Human platelet Osteonectin. SPARC produced by various cells grown in culture. Cross reacts with Rat Osteonectin.Aff. Const.:5 x 10e-9 M

Purification: Ammonium Sulfate Precipitation, Ion Exchange Chromatography and Gel Filtration.

Immunogène: Crude bone extract containing native Osteonectin.

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 10.8 mg/mL (OD280 nm, E1% = 14)

Buffer: 50 % Glycerol/H2O (vol/vol), None

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C/-80 °C

Stockage commentaire: Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.

Détails sur SPARC

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Autre désignation: SPARC / Osteonectin

Sujet: SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules. Tumor growth is enhanced in mice lacking SPARC due to changes in the ECM that create a more permissive environment for tumor progression.Synonyms: BM-40, Basement-membrane protein 40, ON, Secreted protein acidic and rich in cysteine

ID gène: 6678

NCBI Accession: NP_003109

UniProt: P09486

Pathways: Autophagy