Aconitase 1 anticorps (pSer138)

N° du produit ABIN1531675

Cet anticorps Lapin Polyclonal détecte spécifiquement Aconitase 1 dans IHC, ELISA et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Aconitase 1 anticorps (pSer138) (ABIN1531675)

Antigène: Aconitase 1 (ACO1)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Aconitase 1 est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-Aconitase 1 anticorps

Épitope: AA 106-155, pSer138

Fonction: IRP-1 (phospho Ser138) Antibody

Specificité: Phospho-IRP-1 (S138) Polyclonal Antibody detects endogenous levels of IRP-1 protein only when phosphorylated at S138.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pureté: > 95 %

Immunogène: The antiserum was produced against synthesized peptide derived from human IREB1 around the phosphorylation site of Ser138. AA range:106-155

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C/1 year

Date de péremption: 12 months

Détails sur Aconitase 1

Antigène: Aconitase 1 (ACO1)

Autre désignation: IREB1

Sujet:

Synonyms: ACO1; IREB1; Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1

Background: The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct

Gene Name: ACO1

Protein Name: Cytoplasmic aconitate hydratase

Poids moléculaire: 98 kDa

ID gène: 48

OMIM: 100880

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis