CEP55 anticorps (AA 81-130)

N° du produit ABIN1534753

L’anticorps anti-CEP55 Polyclonal Lapin est utilisé pour la détection de CEP55 dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB, ELISA, IHC et IF.

Aperçu rapide pour CEP55 anticorps (AA 81-130) (ABIN1534753)

Antigène: CEP55 (Centrosomal Protein 55kDa (CEP55))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CEP55 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-CEP55 anticorps

Épitope: AA 81-130

Fonction: CEP55 Antibody

Specificité: CEP55 Polyclonal Antibody detects endogenous levels of CEP55 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pureté: > 95 %

Immunogène: The antiserum was produced against synthesized peptide derived from human CEP55. AA range:81-130

Isotype: IgG

Information d'application

Indications d'application: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C/1 year

Date de péremption: 12 months

Détails sur CEP55

Antigène: CEP55 (Centrosomal Protein 55kDa (CEP55))

Autre désignation: CEP55

Sujet:

Synonyms: CEP55, C10orf3, URCC6, Centrosomal protein of 55 kDa, Cep55, Up-regulated in colon cancer 6

Background: function:Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.,PTM:There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.,subcellular location:Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.,subunit:Homodimer. Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1. Interacts with AKAP9, the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT, the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PDCD6IP, the interaction is direct, CEP55 binds PDCD6IP in a 2:1 stoechiometry, PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101, TSG101 competes with PDCD6IP for the same binding site, interaction is required for cytokinesis but not for viral budding. Interacts with FAM125A, VPS37B, VPS37C and VPS28.,tissue specificity:Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.,

Gene Name: CEP55

Protein Name: Centrosomal protein of 55 kDa

Poids moléculaire: 54 kDa

ID gène: 55165

OMIM: 610000

UniProt: Q53EZ4