CD3EAP anticorps (AA 441-490)

N° du produit ABIN1534844

Cet anticorps anti-CD3EAP Polyclonal Lapin (ABIN1534844) détecte spécifiquement CD3EAP dans WB, ELISA, IHC et IF. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour CD3EAP anticorps (AA 441-490) (ABIN1534844)

Antigène: CD3EAP (CD3e Molecule, epsilon Associated Protein (CD3EAP))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD3EAP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-CD3EAP anticorps

Épitope: AA 441-490

Fonction: CD3EAP Antibody

Specificité: CD3EAP Polyclonal Antibody detects endogenous levels of CD3EAP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pureté: > 95 %

Immunogène: The antiserum was produced against synthesized peptide derived from human CD3EAP. AA range:441-490

Isotype: IgG

Information d'application

Indications d'application: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C/1 year

Date de péremption: 12 months

Détails sur CD3EAP

Antigène: CD3EAP (CD3e Molecule, epsilon Associated Protein (CD3EAP))

Autre désignation: CD3EAP

Sujet:

Synonyms: CD3EAP, ASE1, CAST, PAF49, DNA-directed RNA polymerase I subunit RPA34, A34.5, Antisense to ERCC-1 protein, ASE-1, CD3-epsilon-associated protein, CAST, CD3E-associated protein, RNA polymerase I-associated factor PAF49

Background: caution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling.,function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.,function:Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.,miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function.,PTM:Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.,PTM:Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eukaryotic RPA34 RNA polymerase subunit family.,subcellular location:Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.,subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E.,

Gene Name: CD3EAP

Protein Name: DNA-directed RNA polymerase I subunit RPA34

Poids moléculaire: 54 kDa

ID gène: 10849

OMIM: 107325

UniProt: O15446