EIF3F anticorps (AA 81-130)

N° du produit ABIN1534899

Cet anticorps Lapin Polyclonal détecte spécifiquement EIF3F dans WB, IHC, ELISA et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour EIF3F anticorps (AA 81-130) (ABIN1534899)

Antigène: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF3F est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-EIF3F anticorps

Épitope: AA 81-130

Fonction: eIF3ε Antibody

Specificité: EIF3ε Polyclonal Antibody detects endogenous levels of eIF3ε protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pureté: > 95 %

Immunogène: The antiserum was produced against synthesized peptide derived from human EIF3F. AA range:81-130

Isotype: IgG

Information d'application

Indications d'application: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C/1 year

Date de péremption: 12 months

Détails sur EIF3F

Antigène: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Autre désignation: EIF3F

Sujet:

Synonyms: EIF3F, EIF3S5, Eukaryotic translation initiation factor 3 subunit F, eIF3f, Deubiquitinating enzyme eIF3f, Eukaryotic translation initiation factor 3 subunit 5, eIF-3-epsilon, eIF3 p47

Background: function:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,PTM:Phosphorylated. Phosphorylation is enhanced upon serum stimulation.,similarity:Belongs to the eIF-3 subunit F family.,similarity:Contains 1 MPN (JAB/Mov34) domain.,subunit:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I, module B is composed of EIF3F, EIF3H, and EIF3M, and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.,

Gene Name: EIF3F

Protein Name: Eukaryotic translation initiation factor 3 subunit F

Poids moléculaire: 37 kDa

ID gène: 8665

OMIM: 603914

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization