Podoplanin anticorps

N° du produit ABIN155169

Détail du produit anti-Podoplanin anticorps

Antigène: Podoplanin (PDPN)

Reactivité: Souris

Hôte: Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp Podoplanin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: The monoclonal antibody will detect Podoplanin on the surface of lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. The antibody does not cross-react with Human Podoplanin.

Purification: Protein G Chromatography

Immunogène: Murine thymic stromal cell line

Clone: RTD4E10

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (use diluted 1-10 μg/mL IgG). Works well with paraffin sections. Western blot (use 1-10 μg/mL IgG, will detect a 36-40 kDa band). Immunoprecipitation (use 1-5 μg IgG per 1 mLlysate or reaction volume).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.4, without preservatives or stabilizers

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. After reconstitution store the antibody (in aliquots) at -20 °C.

Références pour anticorps anti-Podoplanin (ABIN155169)

Uhrin, Zaujec, Breuss, Olcaydu, Chrenek, Stockinger, Fuertbauer, Moser, Haiko, Fässler, Alitalo, Binder, Kerjaschki: "Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation." dans: Blood, Vol. 115, Issue 19, pp. 3997-4005, (2010) (PubMed).

DAmico, Jones, Nye, Sapienza, Ramjuan, Reynolds, Robinson, Kostourou, Martinez, Aubyn, Grose, Thomas, Spencer-Dene, Zicha, Davies, Tybulewicz, Hodivala-Dilke: "Regulation of lymphatic-blood vessel separation by endothelial Rac1." dans: Development (Cambridge, England), Vol. 136, Issue 23, pp. 4043-53, (2009) (PubMed).

NOYES, DOMM, WILLSLC: "Regulation of erythropoiesis. I. Erythropoietin assay as a clinical tool." dans: Blood, Vol. 20, pp. 9-18 (PubMed).

Détails sur Podoplanin

Antigène: Podoplanin (PDPN)

Autre désignation: Podoplanin (PDPN Produits)

Synonymes: anticorps PDPN, anticorps AGGRUS, anticorps GP36, anticorps GP40, anticorps Gp38, anticorps HT1A-1, anticorps OTS8, anticorps PA2.26, anticorps T1A, anticorps T1A-2, anticorps OTS-8, anticorps RANDAM-2, anticorps T1-alpha, anticorps T1a, anticorps T1alpha, anticorps E11, anticorps RTI40, anticorps podoplanin, anticorps PDPN, anticorps Pdpn

Sujet: Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

ID gène: 14726

NCBI Accession: NP_034459

UniProt: Q62011

Pathways: Dicarboxylic Acid Transport