Trx Tag anticorps

N° du produit ABIN1573883

Détail du produit

Antigène: Trx Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA

Specificité: This antibody reacts with the Thioredoxin fusion protein from E. coli and human. Not yet tested in other species.

Réactivité croisée (Details): Reactivity to other species is not tested yet.

Purification: Protein G affinity column

Immunogène: Purified recombinant Trx-tag fusion protein

Clone: 4C12H10

Isotype: IgG2a

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.1-1.0 µg/mL
Western blot: 1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 mL WB with 2ml of PBST).Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: Trx Tag

Autre désignation: Trx-Tag

Classe de substances: Tag

Sujet: Mouse Anti-Trx-tag Monoclonal Antibody reacts with the 109-aa Trx-tag sequence encoded by the PET-32a expression vectors from Novagen. Mouse Anti-Trx-tag Monoclonal Antibody is suitable for detecting fusion proteins that contain a Trx-tag.