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Protein C Tag anticorps

WB, ELISA Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1573910
  • Antigène Tous les produits Protein C Tag
    Protein C Tag
    Hôte
    • 2
    • 1
    Lapin
    Clonalité
    • 2
    • 1
    Polyclonal
    Conjugué
    • 3
    Cet anticorp Protein C Tag est non-conjugé
    Application
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA
    Specificité
    This Antibody recognizes C-terminal, N-terminal, and internal Protein C tagged fusion proteins.
    Purification
    Immunoaffinity chromatography
    Immunogène
    Protein C epitope tag peptide CEDQVDPRLIDGK conjugated - KLH
    Isotype
    IgG
  • Indications d'application
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm X 8 cm membrane.Other applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.02 %Sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Wong, Bienzle, Hayes, Taylor, Wood: "Purification of protein C from canine plasma." dans: BMC veterinary research, Vol. 10, pp. 251, (2015) (PubMed).

    Wolf, Reimer, Schuck, Rüder, Gerlach, Müller, Otto, Dörken, Rehm: "Role of EBAG9 protein in coat protein complex I-dependent glycoprotein maturation and secretion processes in tumor cells." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 10, pp. 4000-19, (2010) (PubMed).

  • Antigène
    Protein C Tag
    Abstract
    Protein C Tag Produits
    Classe de substances
    Tag
    Sujet
    Rabbit Anti-Protein C (HPC4)-tag is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH7.4, containing 0.02% Sodium azide.
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