DDDDK Tag anticorps

N° du produit ABIN1573914

Cet anticorps Lapin Polyclonal détecte spécifiquement DDDDK Tag dans WB et ELISA.

Aperçu rapide pour DDDDK Tag anticorps (ABIN1573914)

Antigène: DDDDK Tag

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DDDDK Tag est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-DDDDK Tag anticorps

Séquence: DDDDK

Specificité: This Antibody recognizes the enterokinase cleavage site and specifically binds to fusion proteins containing C-terminal, N-terminal, and internal xxxDDDDK epitope tags, such as Xpress tag or Flag tag

Purification: Immunoaffinity chromatography

Immunogène: A synthetic epitope peptide xxxDDDDK conjugated - KLH. This sequence represents the enterokinase cleavage site (ECS).

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using the ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) is recommended. 5 µg of this antibody is mixed with 10 mL of WB solution for any 8 cm X 8 cm membrane.Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Détails sur DDDDK Tag

Antigène: DDDDK Tag

Autre désignation: DDDDK-Tag

Classe de substances: Tag

Sujet: Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The xxxDDDDK-tag is a small size and high hydrophilicity epitope tag containing an enterokinase cleavage site that allows it to be completely removed from the fusion proteins.The Rabbit Anti-DDDDK-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide containing xxxDDDDK epitope conjugated to KLH.This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml.