TUBB anticorps

N° du produit ABIN1574254

Détail du produit anti-TUBB anticorps

Antigène: TUBB (Tubulin, beta (TUBB))

Reactivité: Humain, Souris, Rat, Lapin, Porc, Poulet, Boeuf (Vache), Poisson, Chévre

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBB est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificité: Beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.

Réactivité croisée (Details): beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.

Purification: Protein A affinity column

Immunogène: purified human recombinant full-length β-tubulin protein

Clone: 2G7D4

Isotype: IgG1

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL Flow cytometry:1 µg for 1 x 106 cells

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-TUBB (ABIN1574254)

Fisher, Resnick, De, Acevedo, Lu, Schroeder, Nicholson: "Cyclic cis-Locked Phospho-Dipeptides Reduce Entry of AβPP into Amyloidogenic Processing Pathway." dans: Journal of Alzheimer's disease : JAD, Vol. 55, Issue 1, pp. 391-410, (2016) (PubMed).

Huang, Nishi, Li, Ho, Dong, Chang, Chang: "Acyl-coenzyme A:cholesterol acyltransferase 1 - significance of single-nucleotide polymorphism at residue 526 and the role of Pro347 near the fifth transmembrane domain." dans: The FEBS journal, Vol. 281, Issue 7, pp. 1773-83, (2014) (PubMed).

Sukthankar, Avila, Whitaker, Iwamoto, Morgenstern, Apostolidis, Liu, Hanzlik, Dadachova, Tomich: "Branched amphiphilic peptide capsules: cellular uptake and retention of encapsulated solutes." dans: Biochimica et biophysica acta, Vol. 1838, Issue 9, pp. 2296-305, (2014) (PubMed).

Infanger, Cho, Lopez, Mohanan, Liu, Gursel, Boockvar, Fischbach: "Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche." dans: Cancer research, Vol. 73, Issue 23, pp. 7079-89, (2013) (PubMed).

Wang, Chatterjee, Jeon, Akerman, Vander Heiden, Cantley, Krainer: "Exon-centric regulation of pyruvate kinase M alternative splicing via mutually exclusive exons." dans: Journal of molecular cell biology, Vol. 4, Issue 2, pp. 79-87, (2012) (PubMed).

Détails sur TUBB

Antigène: TUBB (Tubulin, beta (TUBB))

Autre désignation: beta-Tubulin (TUBB Produits)

Synonymes: anticorps M40, anticorps OK/SW-cl.56, anticorps TUBB1, anticorps TUBB5, anticorps XLOT, anticorps m40, anticorps ok/sw-cl.56, anticorps tubb1, anticorps tubb5, anticorps TUBB2A, anticorps TUBB2B, anticorps TUBB, anticorps 1t, anticorps B1t, anticorps BETA 56D, anticorps CG9277, anticorps DTB2, anticorps Dmbeta1, anticorps Dmel\\CG9277, anticorps T, anticorps Tub, anticorps Tubulin, anticorps beta, anticorps beta-Tub, anticorps beta-Tub56D, anticorps beta-tub, anticorps beta-tubulin56D, anticorps beta1, anticorps beta1-Tubulin, anticorps beta1-tub, anticorps beta1Tub, anticorps beta1t, anticorps beta1tub, anticorps beta56D, anticorps betaTub, anticorps betaTub1, anticorps beta[[1]] tubulin, anticorps beta[[1]]-tubulin, anticorps betatub(56D), anticorps 143391_i_at, anticorps 3t, anticorps B3t, anticorps BETA 60D, anticorps CG3401, anticorps D.m.BETA-60D, anticorps DTB3, anticorps Dmbeta3, anticorps Dmel\\CG3401, anticorps Tub60D, anticorps beta-Tub60D, anticorps beta-Tub6D, anticorps beta3, anticorps beta3 TU, anticorps beta3-Tub, anticorps beta3-tubulin, anticorps beta3TUB, anticorps beta3Tub, anticorps beta3t, anticorps beta60C, anticorps betaTub3, anticorps betaTub60C, anticorps beta[[3]] tubulin, anticorps beta[[3]]-Tub, anticorps beta[[3]]-tubulin, anticorps betatub60D, anticorps p50, anticorps p50/tubulin, anticorps p53, anticorps p53/tubulin, anticorps 2t, anticorps B2t, anticorps BETA 85D, anticorps BETA2, anticorps CG9359, anticorps D.m.BETA-85D, anticorps DTB4, anticorps Dmbeta2, anticorps Dmel\\CG9359, anticorps beta(2)Tu, anticorps beta(2)Tub, anticorps beta-Tub85D, anticorps beta-tub85D, anticorps beta2, anticorps beta2-tub, anticorps beta2t, anticorps beta2tub, anticorps beta85D, anticorps betaTub2, anticorps beta[[2]]-tubulin, anticorps ms(3)KK[D], anticorps 4t, anticorps B4t, anticorps BETA 98B, anticorps CG4869, anticorps DTB1, anticorps Dmbeta4, anticorps Dmel\\CG4869, anticorps beta-Tub97EF, anticorps beta4, anticorps beta4t, anticorps beta97F, anticorps betaTub4, anticorps betaTub98BC, anticorps betaTub98C, anticorps Tub1, anticorps bmtub1, anticorps Tub3, anticorps bmtub3, anticorps Tub2, anticorps bmtub2, anticorps Tub4, anticorps bmtub4, anticorps LOC100101153, anticorps tubulin beta class I, anticorps putative cobalt-zinc-cadmium outer membrane resistance protein, anticorps Tubulin beta chain, anticorps tubulin beta-2A chain, anticorps beta-Tubulin at 56D, anticorps beta-Tubulin at 60D, anticorps beta-Tubulin at 85D, anticorps beta-Tubulin at 97EF, anticorps beta-tubulin, anticorps tubulin beta 6 class V, anticorps beta tubulin, anticorps tubulin beta class I L homeolog, anticorps tubulin, beta 5 class I, anticorps TUBB, anticorps czcC, anticorps tbb-6, anticorps tubb, anticorps LOC462399, anticorps betaTub56D, anticorps betaTub60D, anticorps betaTub85D, anticorps betaTub97EF, anticorps Tub1, anticorps Tub3, anticorps Tub2, anticorps Tub4, anticorps TVAG_525430, anticorps TVAG_062880, anticorps TVAG_456920, anticorps LOC100101153, anticorps TUBB6, anticorps Tb927.1.2330, anticorps Tb927.1.2350, anticorps Tb927.1.2370, anticorps Tb927.1.2390, anticorps tubb.L, anticorps Tubb, anticorps LOC693049, anticorps LOC443015, anticorps Tubb5

Sujet: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. It is a dimer of alpha (alpha) and beta (beta) chains. beta-tubulin is ubiquitously expressed with highest levels in spleen, thymus and immature brain and it has a highly acidic C-terminal region which may bind cations such as calcium. This antibody can be used as a loading control on Western blots. beta-tubulin Antibody (2G7D4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human recombinant full-length beta-tubulin protein.

Pathways: Dynamique des Microtubules, M Phase