Avi-Tag anticorps

N° du produit ABIN1574261

Cet anticorps Souris Monoclonal détecte spécifiquement Avi-Tag dans WB, ELISA, ICC, FACS et IF. Il a été cité dans 4+ publications.

Aperçu rapide pour Avi-Tag anticorps (ABIN1574261)

Antigène: Avi-Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Avi-Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 1D11D10

Détail du produit anti-Avi-Tag anticorps

Séquence: GLNDIFEAQKIEWHE

Specificité: Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.

Réactivité croisée (Details): Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.

Purification: Protein A affinity column

Immunogène: Avi peptide conjugated - KLH

Isotype: IgG2a

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL FACS: 1-3 µg for 1 x 106 cells ICC/IF: 1-3 µg/mL ELISA: 0.02-0.05 µg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-Avi-Tag (ABIN1574261)

Nogal, McCoy, van Gils, Cottrell, Voss, Andrabi, Pauthner, Liang, Messmer, Nedellec, Shin, Turner, Ozorowski, Sanders, Burton, Ward: "HIV envelope trimer-elicited autologous neutralizing antibodies bind a region overlapping the N332 glycan supersite." dans: Science advances, Vol. 6, Issue 23, pp. eaba0512, (2020) (PubMed).

Sheridan, Fong, DAlessandro, Bentley: "Widespread Backtracking by RNA Pol II Is a Major Effector of Gene Activation, 5' Pause Release, Termination, and Transcription Elongation Rate." dans: Molecular cell, Vol. 73, Issue 1, pp. 107-118.e4, (2019) (PubMed).

Hill, Martinko, Nguyen, Wells: "Human antibody-based chemically induced dimerizers for cell therapeutic applications." dans: Nature chemical biology, Vol. 14, Issue 2, pp. 112-117, (2019) (PubMed).

Iovino, Orihuela, Moorlag, Molema, Bijlsma: "Interactions between blood-borne Streptococcus pneumoniae and the blood-brain barrier preceding meningitis." dans: PLoS ONE, Vol. 8, Issue 7, pp. e68408, (2014) (PubMed).

Détails sur Avi-Tag

Antigène: Avi-Tag

Autre désignation: Avi Tag

Classe de substances: Tag

Sujet: Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Avi Tag Antibody is a useful tool in analysis of Avi fusion proteins.