ALDOC anticorps (C-Term)

N° du produit ABIN1580409

Cet anticorps anti-ALDOC est un anticorps Souris Monoclonal détectant ALDOC dans WB, IHC, IF et ICC. Adapté pour Humain, Souris, Rat, Boeuf (Vache), Porc et Mammifères.

Aperçu rapide pour ALDOC anticorps (C-Term) (ABIN1580409)

Antigène: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Mammifères

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ALDOC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1A1

Détail du produit anti-ALDOC anticorps

Épitope: C-Term

Purification: affinity purified antibody

Isotype: IgG1

Information d'application

Indications d'application: The antibody solution can be used at dilutions 1:1,000-2,000 for immunofluorescence. For western blots try at 1:2,000-5,000. A suitable control tissue is brain cell lysates.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4°C short term or -20°C long term.

Détails sur ALDOC

Antigène: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Autre désignation: Aldolase C

Sujet: Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde 3-phosphate or glyceraldehyde, respectively. Three aldolase isozymes are found in mammals, specifically aldolases A, B, and C, each of which is encoded by a separate gene.Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia. Aldolase B is considered to be a liver-specific enzyme and it is transcriptionally activated by signals from hormones and dietary factors. In the adult, aldolase C is the brain-specific isozyme, with low but detectable activity in fetal tissues. Aldolase C shares 81% amino acid identity with aldolase A and 70% identity with aldolase B. Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater. In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that aldolase A and C are expressed in complementary cell types: aldolase A mRNA is found in neurons, aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cells. Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific. MCA-1A1 was raised against C-terminal 23 amino acids of the human aldolase C protein, the sequence is KYEGSGEDGGAAAQSLYIANHAY. The HGNC name for this protein is ALDOC.