GAP43 anticorps

N° du produit ABIN1580420

Détail du produit anti-GAP43 anticorps

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivité: Rat, Souris

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp GAP43 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: IgY preparation

Isotype: IgY

Information d'application

Indications d'application: The IgY solution can be diluted to 1:500-1,000 for immunofluorescence staining and 1:1,000-5,000 for western blotting. On western blots look for a major band at 43 kDa, depending on the species.

Restrictions: For Research Use only

Stockage

Format: Liquid

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4°C short term or -20°C long term.

Détails sur GAP43

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Autre désignation: GAP43 (GAP43 Produits)

Synonymes: anticorps B-50, anticorps PP46, anticorps Basp2, anticorps GAP-43, anticorps b-50, anticorps gap-43, anticorps gap43b, anticorps pp46, anticorps cb310, anticorps gap, anticorps gap43, anticorps gap43a, anticorps growth associated protein 43, anticorps growth associated protein 43 L homeolog, anticorps growth associated protein 43 S homeolog, anticorps GAP43, anticorps Gap43, anticorps gap43.L, anticorps gap43, anticorps gap43.S

Sujet: GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve. Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14KDa (to perform such calculations yourself see this link). GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and which run anomolously on SDS-PAGE. Other examples are microtubule associated proteins MAP2 and tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.