GLN1 anticorps (Biotin)

N° du produit ABIN1607650

N° du produit (Fournisseur): 200-106-238-0100

L’anticorps Chèvre Polyclonal anti-GLN1 a été validé pour WB, IP et ELISA. Il convient pour détecter GLN1 dans des échantillons de Brevibacterium.

Aperçu rapide pour GLN1 anticorps (Biotin) (ABIN1607650)

Antigène: GLN1 (Glutamine Synthetase (GLN1))

Reactivité: Brevibacterium

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GLN1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Détail du produit anti-GLN1 anticorps (Biotin)

N° du produit (Fournisseur): 200-106-238-0100

Fournisseur: Rockland

Fonction: Glutamine Synthetase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial].

Attributs du produit: Synonyms: goat anti-Glutamine Synthetase Antibody biotin Conjugation, biotin Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase

Purification: Anti-Glutamine Synthetase (microbial) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Glutamine Synthetase [Microbial]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Glutamine Synthetase (microbial) antibody has been assayed against 1.0 μg of Glutamine Synthetase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur GLN1

Antigène: GLN1 (Glutamine Synthetase (GLN1))

Autre désignation: Glutamine Synthetase

Sujet: Background: Glutamine Synthetase is a key enzyme in the metabolism of nitrogen. Glutamine synthetase catalyzes an ATP-dependent condensation reaction between ammonia and glutamate to yield glutamine. Glutamine is a key builder of proteins as well as a vehicle to deliver nitrogen atoms to enzymes that build molecules dependent on nitrogen. Glutamine Synthetase from a microbial source is composed of twelve subunits that each house an active site. During the reaction of glutamine synthetase, the active sites bind ammonia and glutamate, as well as an ATP molecule to power the reaction. Negative feedback regulation is provided by the active sites ability to weakly bind other molecules and once their concentrations rise too high, the enzyme shuts off. Glutamine Synthetase has applications in neuroscience due to location in astrocytes within the brain and fluctuations in glutamine synthetase can detrimentally effect the astrocytes. Anti-Glutamine Synthetase (Microbial) Antibody is ideal for investigators in Molecular Biology, Neuroscience, and Enzymology.

ID gène: 3345165

UniProt: Q79VE3

Pathways: Positive Regulation of Peptide Hormone Secretion