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GLN1 anticorps (Biotin)

GLN1 Reactivité: Brevibacterium WB, IP, ELISA Hôte: Chèvre Polyclonal Biotin
N° du produit ABIN1607650
  • Antigène Voir toutes GLN1 Anticorps
    GLN1 (Glutamine Synthetase (GLN1))
    Reactivité
    • 43
    • 35
    • 19
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Brevibacterium
    Hôte
    • 45
    • 7
    • 2
    • 1
    Chèvre
    Clonalité
    • 48
    • 7
    Polyclonal
    Conjugué
    • 21
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp GLN1 est conjugé à/à la Biotin
    Application
    • 46
    • 26
    • 15
    • 15
    • 13
    • 13
    • 8
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    Réactivité croisée (Details)
    Cross reactivity against Glutamine Synthetase from other sources is unknown.
    Pureté
    Anti-Glutamine Synthetase (microbial) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial].
    niveau d'endotoxine
    Low Endotoxin : No
    Immunogène
    Glutamine Synthetase [Microbial]
    Immunogen Type: Native Protein
    Isotype
    IgG
    Top Product
    Discover our top product GLN1 Anticorps primaire
  • Indications d'application
    Anti-Glutamine Synthetase (microbial) antibody has been assayed against 1.0 µg of Glutamine Synthetase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Conseil sur la manipulation
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Date de péremption
    Expiration date is one (1) year from date of opening.
  • Antigène
    GLN1 (Glutamine Synthetase (GLN1))
    Autre désignation
    Glutamine Synthetase (GLN1 Produits)
    Synonymes
    anticorps GLNS, anticorps GS, anticorps PIG43, anticorps PIG59, anticorps Glns, anticorps glutamate-ammonia ligase, anticorps glutamate-ammonia ligase (glutamine synthetase), anticorps glutamine synthetase, anticorps GLUL, anticorps Glul, anticorps GLN1
    Sujet
    Glutamine Synthetase is a key enzyme in the metabolism of nitrogen. Glutamine synthetase catalyzes an ATP-dependent condensation reaction between ammonia and glutamate to yield glutamine. Glutamine is a key builder of proteins as well as a vehicle to deliver nitrogen atoms to enzymes that build molecules dependent on nitrogen. Glutamine Synthetase from a microbial source is composed of twelve subunits that each house an active site. During the reaction of glutamine synthetase, the active sites bind ammonia and glutamate, as well as an ATP molecule to power the reaction. Negative feedback regulation is provided by the active sites ability to weakly bind other molecules and once their concentrations rise too high, the enzyme shuts off. Glutamine Synthetase has applications in neuroscience due to location in astrocytes within the brain and fluctuations in glutamine synthetase can detrimentally effect the astrocytes. Anti-Glutamine Synthetase (Microbial) Antibody is ideal for investigators in Molecular Biology, Neuroscience, and Enzymology. Synonyms: Glutamine synthetase EC=6.3.1.2
    ID gène
    3345165
    UniProt
    Q79VE3
    Pathways
    Positive Regulation of Peptide Hormone Secretion
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