CA1 anticorps (Biotin)

N° du produit ABIN1607667

N° du produit (Fournisseur): 200-1654-0100

Cet anticorps Chèvre Polyclonal détecte spécifiquement CA1 dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour CA1 anticorps (Biotin) (ABIN1607667)

Antigène: CA1 (Carbonic Anhydrase I (CA1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CA1 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-CA1 anticorps (Biotin)

N° du produit (Fournisseur): 200-1654-0100

Fournisseur: Rockland

Fonction: Carbonic Anhydrase I Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes].

Attributs du produit: Synonyms: goat anti-Carbonic Anhydrase I Antibody biotin Conjugation, biotin Conjugated goat anti-Carbonic Anhydrase I Antibody, Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody

Purification: Anti-CARBONIC ANHYDRASE I (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Carbonic Anhydrase I [Human Erythrocytes]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Carbonic anhydrase I Biotin Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Carbonic Anhydrase I in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by researcher.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur CA1

Antigène: CA1 (Carbonic Anhydrase I (CA1))

Autre désignation: CA1

Sujet: Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. Carbonic Anhydrase 1 (CA1) is closely related to Carbonic Anhydrase 2 (CA2) and Carbonic Anhydrase 3 (CA3), and it is a cytosolic protein that is found at the highest level in erythrocytes. Anti-Carbonic anhydrase I antibody is ideal for investigators involved in serum protein components.

ID gène: 759

NCBI Accession: NP_001122301

UniProt: P00915