Dextranase anticorps (Biotin)

N° du produit ABIN1607709

Détail du produit anti-Dextranase anticorps (Biotin)

Antigène: Dextranase

Reactivité: Penicillium sp.

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Dextranase est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Dextranase from other sources is unknown.

Pureté: Anti-Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Dextranase [Penicillium species] DEX
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Dextranase has been assayed against 1.0 µg of Dextranase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:14.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Dextranase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:14.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Dextranase

Antigène: Dextranase

Abstract: Dextranase Produits

Synonymes: anticorps dextranase, anticorps Dextranase, anticorps Teth39_0264, anticorps Teth514_0757, anticorps VDBG_04792, anticorps Thet_2161, anticorps Thebr_0271

Sujet: Anti-Dextranase antibody is a transferase enzyme which catalyzes the synthesis of dextran by engaging in the endohydrolysis of 1, 6 alpha-D-glucosidic linkages in dextran. Belonging to the glycosyl hydrolase 49 family, Anti-Destranase antibody is ideal for researchers interested in Metabolism and Signal Transduction research. Synonyms: Dextranase Alpha-1,6-glucan-6-glucanohydrolase DEX

UniProt: P48845