CPY anticorps (Biotin)

N° du produit ABIN1607713

Détail du produit anti-CPY anticorps (Biotin)

Antigène: CPY (Carboxypeptidase Y (CPY))

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CPY est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Carboxypeptidase Y from other sources may occur but has not been specifically determined.

Pureté: Anti-Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker’s Yeast].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Carboxypeptidase Y [Baker’s Yeast]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Carboxypeptidase Y has been assayed against 1.0 µg of Carboxypeptidase Y in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur CPY

Antigène: CPY (Carboxypeptidase Y (CPY))

Autre désignation: Carboxypeptidase Y (CPY Produits)

Sujet: Anti-Carboxypeptidase Y antibody is a participant in degrading small peptides. Carboyxpeptidase Y preferentially digests peptides containing aliphatic or hydrophobic residue in P1’ position, as well as methionine, leucine, or phenylalanine in P1 position of ester substrate. A member of the peptidase S10 family, Anti-Carboxypeptidase Y is ideal for researchers interested in Metabolism, Signal Transduction, and Cell Biology research. Synonyms: Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody

ID gène: 855343

UniProt: P00729