Amylase, alpha anticorps (Biotin)

N° du produit ABIN1607777

N° du produit (Fournisseur): 200-4645-0100

Cet anticorps anti-Amylase, alpha est un anticorps Lapin Polyclonal détectant Amylase, alpha dans WB, ELISA et IP. Adapté pour Bacillus amyloliquefaciens.

Aperçu rapide pour Amylase, alpha anticorps (Biotin) (ABIN1607777)

Antigène: Amylase, alpha (AMY)

Reactivité: Bacillus amyloliquefaciens

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Amylase, alpha est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-Amylase, alpha anticorps (Biotin)

N° du produit (Fournisseur): 200-4645-0100

Fournisseur: Rockland

Fonction: Alpha Amylase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-Amylase [B.

Attributs du produit: Synonyms: rabbit anti-Alpha Amylase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase

Purification: Anti-Alpha Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: a-Amylase [Bacillus amyloliquefaciens]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Alpha Amylase Biotin Conjugated Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of a-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Amylase, alpha

Antigène: Amylase, alpha (AMY)

Autre désignation: Alpha-amylase

Sujet: Background: Alpha Amylase is a member of the glycosyl hydrolase 13 family, involved primarily in the hydrolysis of biopolymer residues of 1, 4-alpha-D-glucosidic linkages in polysaccharides. It performs its catalytic function in metabolism of carbohydrates. Anti-Alpha Amylase antibody is ideal for investigators involved in Metabolism, Signal Transduction, and Cancer research.

ID gène: 7849308

NCBI Accession: WP_013352208

UniProt: P00692