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Amylase, alpha anticorps (Biotin)

AMY Reactivité: Bacillus amyloliquefaciens WB, ELISA, IP Hôte: Lapin Polyclonal Biotin
N° du produit ABIN1607777
  • Antigène Voir toutes Amylase, alpha (AMY) Anticorps
    Amylase, alpha (AMY)
    Reactivité
    • 9
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Bacillus amyloliquefaciens
    Hôte
    • 16
    • 2
    Lapin
    Clonalité
    • 17
    • 1
    Polyclonal
    Conjugué
    • 9
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Amylase, alpha est conjugé à/à la Biotin
    Application
    • 11
    • 9
    • 8
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Réactivité croisée (Details)
    Cross reactivity against a-Amylase from other sources may occur but has not been specifically determined.
    Pureté
    Anti-Alpha Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-Amylase [B.amyloliquefaciens].
    niveau d'endotoxine
    Low Endotoxin : No
    Immunogène
    a-Amylase [Bacillus amyloliquefaciens]
    Immunogen Type: Native Protein
    Isotype
    IgG
  • Indications d'application
    Anti-Alpha Amylase has been assayed against 1.0 µg of a-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Conseil sur la manipulation
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Date de péremption
    Expiration date is one (1) year from date of opening.
  • Antigène
    Amylase, alpha (AMY)
    Autre désignation
    Alpha Amylase (AMY Produits)
    Synonymes
    anticorps LOC663954, anticorps LOC100136478, anticorps alpha amylase, anticorps alpha-amylase, anticorps LOC663954, anticorps HQ_RS05305, anticorps LOC100136478
    Sujet
    Alpha Amylase is a member of the glycosyl hydrolase 13 family, involved primarily in the hydrolysis of biopolymer residues of 1, 4-alpha-D-glucosidic linkages in polysaccharides. It performs its catalytic function in metabolism of carbohydrates. Anti-Alpha Amylase antibody is ideal for investigators involved in Metabolism, Signal Transduction, and Cancer research. Synonyms: Alpha-amylase EC=3.2.1.1 1,4-alpha-D-glucan glucanohydrolase
    ID gène
    7849308
    UniProt
    P00692
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