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beta-Amylase 1 anticorps (Biotin)

L’anticorps Lapin Polyclonal anti-beta-Amylase 1 a été validé pour WB, ELISA et DB. Il convient pour détecter beta-Amylase 1 dans des échantillons de Sweet potato.
Rockland
N° du produit ABIN1607779
N° du produit (Fournisseur): 200-4647-0100

Aperçu rapide pour beta-Amylase 1 anticorps (Biotin) (ABIN1607779)

Antigène

beta-Amylase 1 (BAM1)

Reactivité

  • 4
  • 1
Sweet potato

Hôte

  • 5
Lapin

Clonalité

  • 5
Polyclonal

Conjugué

  • 3
  • 2
Cet anticorp beta-Amylase 1 est conjugé à/à la Biotin

Application

  • 5
  • 4
  • 2
  • 1
Western Blotting (WB), ELISA, Dot Blot (DB)
  • N° du produit (Fournisseur)

    200-4647-0100

    Fournisseur

    Rockland

    Fonction

    Beta Amylase Antibody Biotin Conjugated

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b-Amylase [Sweet Potato].

    Attributs du produit

    Synonyms: rabbit anti-Beta Amylase Antibody biotin Conjugation, biotin conjugated rabbit anti-Beta Amylase Antibody, 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody

    Purification

    Anti-Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Immunogène

    Immunogen: Beta Amylase [Sweet Potato]

    Immunogen Type: Native Protein

    Isotype

    IgG
  • Indications d'application

    Application Note: Anti-Beta Amylase Biotin Conjugated Antibody has been tested by dot blot and western blot. This product is assayed against 1.0 μg of b-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration

    10 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Date de péremption

    12 months
  • Antigène

    beta-Amylase 1 (BAM1)

    Autre désignation

    BMY1, AMYB

    Sujet

    Background: Anti-Beta Amylase antibody works through the hydrolysis of 1,4 alpha-D-glucosidic linkages in polysaccharides to remove successive maltose units from the non-reducing ends of the chains. Anti-Beta Amylase is often used in the structural analysis of starch and glycogen and is found in the cytoplasmic region. Anti-Beta Amylase antibody is ideal for researchers interested in Metabolism, Signal Transduction, and Cell Biology research.

    UniProt

    P10537
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