DLD anticorps (Biotin)

N° du produit ABIN1607793

Détail du produit anti-DLD anticorps (Biotin)

Antigène: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Reactivité: Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DLD est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Lipoamide Dehydrogenase from other sources may occur but have not been specifically determined.

Pureté: Anti-Lipoamide dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Lipoamide Dehydrogenase [Porcine Heart]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Lipoamide dehydrogenase antibody has been assayed against 1.0 µg of Lipoamide Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur DLD

Antigène: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Autre désignation: Lipoamide Dehydrogenase (DLD Produits)

Synonymes: anticorps DLDD, anticorps DLDH, anticorps E3, anticorps GCSL, anticorps LAD, anticorps PHE3, anticorps AI315664, anticorps AI746344, anticorps wu:fb24b05, anticorps DLD, anticorps DDBDRAFT_0183800, anticorps DDBDRAFT_0216232, anticorps DDB_0183800, anticorps DDB_0216232, anticorps sc:d0402, anticorps dihydrolipoamide dehydrogenase, anticorps dihydrolipoyl dehydrogenase, anticorps deltaD, anticorps DLD, anticorps Dld, anticorps dldh, anticorps AT4G16155, anticorps CND05840, anticorps bfmBC, anticorps GCSL, anticorps LACBIDRAFT_182385, anticorps UREG_06178, anticorps lpd, anticorps TAGG_RS02070, anticorps Arnit_2606, anticorps Mesil_1945, anticorps Trad_2118, anticorps Acear_0640, anticorps Fbal_0372, anticorps Ilyop_1890, anticorps Ftrac_1733, anticorps Ocepr_1753, anticorps Intca_2017, anticorps Deima_0504, anticorps dld

Sujet: Anti-Lipoamide dehydrogenase recognizes the protein lipoamide dehydrogenase. Lipoamide dehydrogenase catalyzes the conversion of lipoamide to dihydrolipoamide with the concomitant oxidation of NADH to NAD+. Lipoamide dehydrogenase is known to play a role in alpha-ketoacid dehydrogenase complexes as well glycine cleavage system. Defects in lipoamide dehydrogenase result in congenital infantile acidosis and maple syrup urine disease. Anti-Lipoamide dehydrogenase is suitable for investigators interested in metabolism, neurology, and cancer. Synonyms: Dihydrolipoyl dehydrogenase, mitochondrial EC=1.8.1.4 Dihydrolipoamide dehydrogenase Lipoamide Dehydrogenase [Porcine Heart]

ID gène: 397129, 118675

UniProt: P09623

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis