Elastase anticorps (Biotin)

N° du produit ABIN1607801

N° du produit (Fournisseur): 200-4668-0100

Cet anticorps anti-Elastase est un anticorps Lapin Polyclonal détectant Elastase dans ELISA et WB. Adapté pour Porc.

Aperçu rapide pour Elastase anticorps (Biotin) (ABIN1607801)

Antigène: Elastase

Reactivité: Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Elastase est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB)

Détail du produit anti-Elastase anticorps (Biotin)

N° du produit (Fournisseur): 200-4668-0100

Fournisseur: Rockland

Fonction: Anti-Elastase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas].

Attributs du produit: Synonyms: rabbit anti-Elastase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Elastase Antibody, Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody

Purification: Anti-Elastase (Rabbit) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Elastase [Porcine Pancreas]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Elastase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Elastase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Elastase

Antigène: Elastase

Sujet: Background: Anti-Elastase antibody detects the Elastase protein. Elastase metabolizes elastin, an elastic fiber, which together with collagen, determines the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A (OmpA) of E. coli and other Gram-negative bacteria. Elastase also has the important immunological role of breaking down Shigella virulence factors. This is accomplished through the cleavage of peptide bonds in the target proteins. The specific peptide bonds cleaved are those on the carboxy side of small, hydrophobic amino acids such as glycine, alanine, and valine. This antibody is suitable for researchers investigating biochemistry and stem cells.

ID gène: 396766

NCBI Accession: NP_998988

UniProt: P00772