AnsB anticorps (Biotin)

N° du produit ABIN1607806

N° du produit (Fournisseur): 200-4671s

Cet anticorps Lapin Polyclonal détecte spécifiquement dans ELISA, WB et DB. Il présente une réactivité envers E. coli.

Aperçu rapide pour AnsB anticorps (Biotin) (ABIN1607806)

Antigène: AnsB

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB), Dot Blot (DB)

Détail du produit

N° du produit (Fournisseur): 200-4671s

Fournisseur: Rockland

Fonction: L-Asparaginase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E.

Attributs du produit: Synonyms: rabbit anti-L-Asparaginase Antibody biotin Conjugation, biotin Conjugated rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody

Purification: Anti-L-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Asparaginase [E. coli]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-L-Asparaginase Biotin Conjugated Antibody has been by ELISA, dot blot, and western blot. This product is assayed against 1.0 μg of Asparaginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,200 to 1:12,800 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500

ELISA Dilution: 1:1,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: AnsB

Sujet: Background: Anti-L-Asparaginase antibody recognizes the protein asparaginase. Asparaginase is responsible for the catalysis of asparagine to L-aspartic acid and ammonia. In food processing, asparaginase is used to prevent the formation of acrylamide, a carcinogen, from the heating of asparagine which causes it to undergo the maillard reaction. Asparaginase is also used in cancer therapy since leukemic cells are unable to produce their own asparagine and therefore, must rely on circulating asparagine. Treatment with asparaginase converts the asparagine to aspartic acid denying the leukemic cells of asparagine, resulting in their death. Anti-L-Asparaginase antibody is suitable to investigators interested in cancer, metabolism, and enzymology.

ID gène: 1039460

UniProt: P00805