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ampC anticorps (Biotin)

L’anticorps Lapin Polyclonal anti-ampC a été validé pour WB, IP et DB. Il convient pour détecter ampC dans des échantillons de Enterobacter cloacae.
Rockland
N° du produit ABIN1607809
N° du produit (Fournisseur): 200-4675-0100

Aperçu rapide pour ampC anticorps (Biotin) (ABIN1607809)

Antigène

Voir toutes ampC Anticorps
ampC (beta-Lactamase (ampC))

Reactivité

  • 5
  • 4
  • 3
Enterobacter cloacae

Hôte

  • 12
Lapin

Clonalité

  • 12
Polyclonal

Conjugué

  • 4
  • 3
  • 3
  • 2
Cet anticorp ampC est conjugé à/à la Biotin

Application

  • 8
  • 4
  • 3
  • 2
Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)
  • N° du produit (Fournisseur)

    200-4675-0100

    Fournisseur

    Rockland

    Fonction

    Penicillinase Antibody Biotin Conjugated

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae].

    Attributs du produit

    Synonyms: rabbit anti-Penicillinase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Penicillinase Antibody, Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody, Cephalosporinase

    Purification

    Anti-Penicillinase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Immunogène

    Immunogen: Penicillinase [Enterobacter cloacae]

    Immunogen Type: Native Protein

    Isotype

    IgG
  • Indications d'application

    Application Note: Anti-Penicillinase Biotin Conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:2,000

    Immunoprecipitation Dilution: 1:100

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration

    10 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Date de péremption

    12 months
  • Antigène

    ampC (beta-Lactamase (ampC))

    Autre désignation

    ampC

    Sujet

    Background: Anti-Penicillinase antibody recognizes the protein penicillinase, a beta lactamase specific to penicillins. Penicillinase hydrolyzes the beta-lactam ring of penicillins destroying their antibacterial potency. Thus, penicillinase is often found in antibiotic-resistant bacteria.

    ID gène

    9125732

    UniProt

    P05364
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