Trypsin Inhibitor anticorps (Biotin)

N° du produit ABIN1607811

Détail du produit anti-Trypsin Inhibitor anticorps (Biotin)

Antigène: Trypsin Inhibitor

Reactivité: Soybean

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Trypsin Inhibitor est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Trypsin Inhibitor from other sources is unknown.

Pureté: Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soy Bean].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Trypsin Inhibitor antibody is suitable for western blotting and for capture ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Trypsin Inhibitor

Antigène: Trypsin Inhibitor

Abstract: Trypsin Inhibitor Produits

Synonymes: anticorps CRISP11, anticorps LCRISP2, anticorps GRMZM2G447785, anticorps cysteine rich secretory protein LCCL domain containing 2, anticorps trypsin inhibitor, anticorps trophoblast Kunitz domain protein 1, anticorps Kazal-type serine proteinase inhibitor 1, anticorps Bowman-Birk type bran trypsin inhibitor, anticorps CRISPLD2, anticorps LOC548021, anticorps TKDP1, anticorps Spi1, anticorps LOC100284348

Sujet: Anti-Trypsin Inhibitor detects trypsin inhibitor from soy bean. Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. Soybean trypsin inhibitor will also inhibit other proteases by a mechanism similar to trypsin. It has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. However, Soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases. Anti-Trypsin Inhibitor Antibody is ideal for investigators involved in Cell Signaling, Biochemistry and Signal Transduction research. Synonyms: 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

ID gène: 547831

UniProt: P01070