Huntingtin anticorps (N-Term)

N° du produit ABIN1607890

N° du produit (Fournisseur): 600-401-j23

Cet anticorps Lapin Polyclonal détecte spécifiquement Huntingtin dans IHC, WB, ELISA et FM. Il présente une réactivité envers Humain, Rat et Souris.

Aperçu rapide pour Huntingtin anticorps (N-Term) (ABIN1607890)

Antigène: Huntingtin (HTT)

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Huntingtin est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Détail du produit anti-Huntingtin anticorps

Épitope: N-Term

N° du produit (Fournisseur): 600-401-j23

Fournisseur: Rockland

Fonction: Huntington Antibody

Réactivité croisée (Details): This antibody is specific towards HTT.

Attributs du produit: Synonyms: rabbit anti-Huntington antibody, rabbit anti-Huntingtin antibody, HD protein, HTT, Huntington Disease Protein, Huntington disease, IT15, LOMARS

Purification: Anti-Huntingtin was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near N-terminus of human Huntington disease protein.

Immunogen Type: Conjugated Peptide

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-Huntingtin antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 0.1 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 6 months

Détails sur Huntingtin

Antigène: Huntingtin (HTT)

Autre désignation: HTT

Sujet: Background: Huntingtin (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. Anti-Huntington antibodies are ideal for researchers interested in Apoptosis, Autophagy, Cytoskeleton, Neurodegeneration, Neuroscience, and Neuronal Cell Markers research.

ID gène: 3064

NCBI Accession: NP_002102

UniProt: P42858

Pathways: Signalisation PI3K-Akt, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport