RFP anticorps (Biotin)

N° du produit ABIN1607901

N° du produit (Fournisseur): 600-906-379

Cet anticorps anti-RFP est un anticorps Poulet Polyclonal détectant RFP dans WB, ELISA, IF et FM. Adapté pour Discosoma. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour RFP anticorps (Biotin) (ABIN1607901)

Antigène: RFP (Red Fluorescent Protein (RFP))

Reactivité: Discosoma

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp RFP est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-RFP anticorps (Biotin)

N° du produit (Fournisseur): 600-906-379

Fournisseur: Rockland

Fonction: RFP Antibody Biotin Conjugated

Réactivité croisée (Details): RFP Antibody was tested by western blot and immunoelectrophoresis against anti-biotin, anti-chicken serum, and RFP.

Attributs du produit: Synonyms: chicken anti-RFP antibody biotin conjugation, biotin conjugated chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Purification: RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Immunogen Type: Recombinant Protein

Information d'application

Indications d'application:

Application Note: Anti-RFP is designed to detect recombinant RFP. This antibody has been tested to detect RFP by immunoblotting and immunofluorescence and is suitable for use in immunohistochemistry. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

Western Blot Dilution: 1:1,000 - 1:3,000

ELISA Dilution: 1:10,000

IF Microscopy Dilution: 1:100

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-RFP (Biotin) (ABIN1607901)

Jo, Xu, Deniz, Cherian, DeVries, Zhu: "Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina." dans: Frontiers in neural circuits, Vol. 12, pp. 66, (2019) (PubMed).

Détails sur RFP

Antigène: RFP (Red Fluorescent Protein (RFP))

Autre désignation: DsRed

Sujet: Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including western blotting.

UniProt: Q9U6Y8