pan Keratin anticorps
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- Antigène Voir toutes pan Keratin (panKRT) Anticorps
- pan Keratin (panKRT)
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp pan Keratin est non-conjugé
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Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond tocytokeratins 4,5,6,8,10,13 and 18.
- Purification
- Concentrated.
- Immunogène
- Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.
- Clone
- B311-1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product panKRT Anticorps primaire
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- Indications d'application
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IHC (FFPE): 1:50-1:100 (Requires high temp. antigen demaking, 10 mM Citrate pH 6.0 boiling tissue for 10-20 mins. followed by cooling at RT for 10-20 mins or protease digestion).
Western blotting: 1:100-1:400
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC, with High temperature antigenic unmasking technique predigestion is recommended with this antibody when using paraffin-embedded tissue sections. - Commentaires
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Cellular Localization: Cytoplasmic.
Recommended Positive Control: Skin, Colonic Carcinoma - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.35 mg/ml.
- Buffer
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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- Antigène
- pan Keratin (panKRT)
- Autre désignation
- Pan Cytokeratin, Pan CK-II (panKRT Produits)
- Synonymes
- anticorps xk70a, anticorps keratin, anticorps keratin S homeolog, anticorps LOC431321, anticorps krt.S
- Poids moléculaire
- 45, 52.5 ,54, 56, 56.5, 58, & 59 kDa
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