IKAP/p150 anticorps (AA 1151-1250) (HRP)

N° du produit ABIN1711362

Cet anticorps anti-IKAP/p150 est un anticorps Lapin Polyclonal détectant IKAP/p150 dans WB, ELISA, IHC (p) et IHC (fro). Adapté pour Souris et Rat.

Aperçu rapide pour IKAP/p150 anticorps (AA 1151-1250) (HRP) (ABIN1711362)

Antigène: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IKAP/p150 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-IKAP/p150 anticorps (HRP)

Épitope: AA 1151-1250

Réactivité croisée: Souris, Rat

Homologie: Human,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human IKAP

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur IKAP/p150

Antigène: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Autre désignation: IKAP

Sujet:

Synonyms: DKFZp781H1425, DYS, Dysautonomia Riley Day syndrome hereditary sensory autonomic neuropathy type III, Elongator complex protein 1, ELP 1, ELP1, ELP1_HUMAN, FD, FLJ12497, IKAP, IkappaB kinase complex associated protein, IkappaB kinase complex-associated protein, ikbkap, IKI 3, IKI3, IKK complex associated protein, IKK complex-associated protein, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein, OTTHUMP00000063889, p150, TOT 1, TOT1

Background: The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in the targeted degradation of IkB (1). IkB kinase alpha (IKK alpha), previously designated CHUK (2), interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation, serines 32 and 36 (3). IKKalpha appears to be critical for NFkB activation in response to proinflammatory cytokines (4). Phosphorylation of the IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1 (5). The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma (also designated NEMO), and each appears to make essential contributions to IkB phosphorylation (6). IKAP (IKK-complex-associated protein) is a protein that acts as a scaffold, interacting with NIK, IKK alpha and IKK beta and assembling them into an active kinase complex (7,8)