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Actin anticorps

Cet anticorps anti-Actin est un anticorps Lapin Polyclonal détectant Actin dans WB et IF. Adapté pour Orge, Arabidopsis thaliana, Soybean et Zea mays. Ce Primary Antibody a été cité dans 1 publication.
N° du produit ABIN1720863

Aperçu rapide pour Actin anticorps (ABIN1720863)

Antigène

Voir toutes Actin Anticorps
Actin

Reactivité

  • 103
  • 79
  • 75
  • 26
  • 22
  • 21
  • 12
  • 11
  • 6
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Orge, Arabidopsis thaliana, Soybean, Zea mays

Hôte

  • 88
  • 43
  • 3
  • 1
  • 1
  • 1
Lapin

Clonalité

  • 81
  • 56
Polyclonal

Conjugué

  • 80
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Cet anticorp Actin est non-conjugé

Application

  • 109
  • 42
  • 33
  • 29
  • 28
  • 27
  • 25
  • 21
  • 18
  • 16
  • 15
  • 3
  • 1
Western Blotting (WB), Immunofluorescence (IF)
  • Specificité

    TAIR: At2g37620, At2g18780, At2g53750, At2g59370, At2g42100, At5g09810, At2g49240, At2g12110, At2g46520

    Réactivité croisée (Details)

    Not reactive in: Chlamydomonas reinhardtii (too high background for this species)

    Homologie

    Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cucumis sativus, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Nicotiana tabacum, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao, Triticum aestivum, Vicia faba

    Purification

    serum

    Immunogène

    ca. 100 amino acids of recombinant actin conserved more than 80 % in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520
  • Indications d'application

    1 : 2500 with standard ECL (WB), 1: 250 (IF)

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution add 50 μL of sterile water.

    Conseil sur la manipulation

    Avoid repeated freeze-thaw cycles.
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    Stock

    -20 °C

    Stockage commentaire

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Zheng, Fang, Pan, Sun, Deng, Grosser, Guo: "iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo." dans: Journal of proteome research, Vol. 13, Issue 6, pp. 2998-3015, (2014) (PubMed).

  • Antigène

    Actin

    Autre désignation

    Actin (ACT)

    Sujet

    Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

    Poids moléculaire

    expected: 41.6 kDa, appearent: 45 kDa

    UniProt

    P0CJ46, Q96292, P0CJ47, P53494, Q8RYC2, P53492, Q96293, P53496, P53497
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