CpxA (N-Term) anticorps

N° du produit ABIN1720869

Cet anticorps anti-CpxA Polyclonal Lapin (ABIN1720869) détecte spécifiquement CpxA dans WB. L’anticorps est réactif avec des échantillons de Yersinia pseudotuberculosis.

Aperçu rapide pour CpxA (N-Term) anticorps (ABIN1720869)

Antigène: CpxA

Reactivité: Yersinia pseudotuberculosis

Hôte: Lapin

Clonalité: Polyclonal

Application: Western Blotting (WB)

Détail du produit

Épitope: N-Term

Fonction: Anti-CpxA | Conjugative plasmid expression

Réactivité croisée (Details): Yersinia pseudotuberculosis
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Purification: Serum

Immunogène: Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag

Information d'application

Indications d'application: 1 : 2000 (WB)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Détail du antigène

Antigène: CpxA

Sujet:

Background: CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.

Additional information: The strains were grown in LB broth until late stationary phase (absorbance at 600nm of ~1,5), To avoid interference from cross-reacting bands, aim to maximize protein separation with the region of 35 to 55 kDa, CpxA could be routinely detected from a 20 μL of sample that was derived from 1 mL of pelleted bacteria resuspended in 200 μL of 1x loading buffer, Re-use of diluted working strength antibody is not recommended

Poids moléculaire: 51.8 | 52-53 kDa