SNAP25 anticorps (AA 192-206)

N° du produit ABIN1742235

Cet anticorps anti-SNAP25 est un anticorps Lapin Polyclonal détectant SNAP25 dans WB, ELISA, IHC, IP et ICC. Adapté pour Humain, Rat, Souris, Poulet, Poisson zèbre (Danio rerio), Hamster et Poisson rouge. Ce Primary Antibody a été cité dans 9+ publications.

Aperçu rapide pour SNAP25 anticorps (AA 192-206) (ABIN1742235)

Antigène: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivité: Humain, Rat, Souris, Poulet, Poisson zèbre (Danio rerio), Hamster, Poisson rouge

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SNAP25 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-SNAP25 anticorps

Épitope: AA 192-206

Specificité: Specific for SNAP 25.

Immunogène: Synthetic peptide IDEANQRATKMLGSG (aa 192-206 in human SNAP 25) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Information d'application

Indications d'application: WB: 1:10000, ICC: 1:500, IHC: 1:200,

Commentaires:

Recognizes the Botulinum neurotoxin A cleavage product with reduced affinity. Does not detect the neurotoxin E cleavage product. Recognizes splice variants SNAP 25A and B. ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200µl H2O.

Agent conservateur: Azide and BSA free

Conseil sur la manipulation: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Stock: 4 °C/-20 °C

Stockage commentaire: Reconstitute and aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized.

Références pour anticorps anti-SNAP25 (ABIN1742235)

Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." dans: eLife, Vol. 5, (2016) (PubMed).

Fossati, Morini, Corradini, Antonucci, Trepte, Edry, Sharma, Papale, Pozzi, Defilippi, Meier, Brambilla, Turco, Rosenblum, Wanker, Ziv, Menna, Matteoli: "Reduced SNAP-25 increases PSD-95 mobility and impairs spine morphogenesis." dans: Cell death and differentiation, Vol. 22, Issue 9, pp. 1425-36, (2015) (PubMed).

Walter, Kurps, de Wit, Schöning, Toft-Bertelsen, Lauks, Ziomkiewicz, Weiss, Schulz, Fischer von Mollard, Verhage, Sørensen: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." dans: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." dans: PLoS ONE, Vol. 8, Issue 7, pp. e68312, (2013) (PubMed).

Young, Franciosi, Spreeuw, Deng, Sanders, Tam, Huang, Singaraja, Zhang, Bissada, Kay, Hayden: "Low levels of human HIP14 are sufficient to rescue neuropathological, behavioural, and enzymatic defects due to loss of murine HIP14 in Hip14-/- mice." dans: PLoS ONE, Vol. 7, Issue 5, pp. e36315, (2012) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." dans: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Camoletto, Vara, Morando, Connell, Marletto, Giustetto, Sassoè-Pognetto, Van Veldhoven, Ledesma: "Synaptic vesicle docking: sphingosine regulates syntaxin1 interaction with Munc18." dans: PLoS ONE, Vol. 4, Issue 4, pp. e5310, (2009) (PubMed).

Johansson, Ericsson, Janson, Beraki, Stanić, Mandic, Wikström, Hökfelt, Ogren, Rozell, Berggren, Bark: "An ancient duplication of exon 5 in the Snap25 gene is required for complex neuronal development/function." dans: PLoS genetics, Vol. 4, Issue 11, pp. e1000278, (2008) (PubMed).

Détails sur SNAP25

Antigène: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Autre désignation: SNAP 25

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport