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SNAP alpha/beta anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement SNAP alpha/beta dans WB, ICC et IP. Il présente une réactivité envers Humain, Souris, Rat et Poisson zèbre (Danio rerio) et a été mentionné dans 3+ publications.
N° du produit ABIN1742242

Aperçu rapide pour SNAP alpha/beta anticorps (ABIN1742242)

Antigène

SNAP alpha/beta

Reactivité

Humain, Souris, Rat, Poisson zèbre (Danio rerio)

Hôte

  • 1
  • 1
Souris

Clonalité

  • 1
  • 1
Monoclonal

Conjugué

  • 2
Cet anticorp SNAP alpha/beta est non-conjugé

Application

Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone

77-2
  • Specificité

    Specific for alpha- and beta SNAP

    Réactivité croisée (Details)

    does not cross-react to gamma SNAP.

    Immunogène

    Recombinant full length rat alpha SNAP.

    Isotype

    IgG1
  • Indications d'application

    WB: 1:1000 up to 1:10000, ICC: 1:500 up to 1:1000,

    Commentaires

    The antibody does not immunoprecipitate the 20 S SNARE-complex.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution add 100µl H2O to get a 1mg/ml solution in PBS.

    Buffer

    PBS.

    Agent conservateur

    Azide and BSA free

    Conseil sur la manipulation

    Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

    Stock

    -20 °C

    Stockage commentaire

    Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.
  • de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." dans: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Hanson, Otto, Barton, Jahn: "The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin." dans: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16955-61, (1995) (PubMed).

  • Antigène

    SNAP alpha/beta
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