Clathrin, Light Chain (AA 156-173) anticorps

N° du produit ABIN1742245

Cet anticorps Souris Monoclonal détecte spécifiquement not_set dans IHC, WB, ICC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat et Poisson zèbre (Danio rerio) et a été mentionné dans 11+ publications.

Aperçu rapide pour Clathrin, Light Chain (AA 156-173) anticorps (ABIN1742245)

Antigène: Clathrin, Light Chain

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 57-4

Détail du produit

Épitope: AA 156-173

Fonction: Mouse anti-Clathrin light chain IgG

Specificité: Specific for both neuronal light chains

Réactivité croisée (Details): Reacts with: human (P09497), rat (P08082), mouse (Q6IRU5), zebrafish. Other species not tested yet.

Purification: purified IgG

Immunogène: Synthetic peptide corresponding to AA 156 to 173 from rat Clathrin light chainB (UniProt Id: P08082)

Isotype: IgG1

Information d'application

Indications d'application: WB:1 : 5000 up to 1 : 10000 , IP:not tested yet ICC:1 : 500 up to 1 : 1000, IHC:1 : 500, IHC-P:1 : 2000,

Commentaires:

Suitable for immunogold electron microscopy.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Stock: -20 °C,-80 °C,4 °C

Stockage commentaire: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

Référence

Neef, Jung, Wong, Reuter, Pangrsic, Chakrabarti, Kügler, Lenz, Nouvian, Boumil, Frankel, Wichmann, Moser: "Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 3, pp. 705-16, (2014) (PubMed).

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." dans: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

Biesemann, Grønborg, Luquet, Wichert, Bernard, Bungers, Cooper, Varoqueaux, Li, Byrne, Urlaub, Jahn, Brose, Herzog: "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." dans: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Hellwig, Hack, Kowalski, Brunne, Jarowyj, Unger, Bock, Junghans, Frotscher: "Role for Reelin in neurotransmitter release." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 7, pp. 2352-60, (2011) (PubMed).

Ferguson, Brasnjo, Hayashi, Wölfel, Collesi, Giovedi, Raimondi, Gong, Ariel, Paradise, Otoole, Flavell, Cremona, Miesenböck, Ryan, De Camilli: "A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis." dans: Science (New York, N.Y.), Vol. 316, Issue 5824, pp. 570-4, (2007) (PubMed).

Krauss, Kukhtina, Pechstein, Haucke: "Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 32, pp. 11934-9, (2006) (PubMed).

Johnson, Brown, Pulliam, Anderson, Johnson: "Synaptic pathology, altered gene expression, and degeneration in photoreceptors impacted by drusen." dans: Investigative ophthalmology & visual science, Vol. 46, Issue 12, pp. 4788-95, (2005) (PubMed).

von Kriegstein, Schmitz: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." dans: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, (2003) (PubMed).

Blumstein, Faundez, Nakatsu, Saito, Ohno, Kelly: "The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 20, pp. 8034-42, (2001) (PubMed).

Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." dans: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

Détail du antigène

Antigène: Clathrin, Light Chain

Autre désignation: Clathrin light chain

Sujet:

Synonyms: Lcb, Cltb

Antigen Information: Clathrin light chain is a small clathrin coat subunit involved in endocytosis

UniProt: P08082