SV2B anticorps (AA 2-17)

N° du produit ABIN1742257

Détail du produit anti-SV2B anticorps

Antigène: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Épitope: AA 2-17

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SV2B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificité: Specific for SV2 B.

Purification: purified IgG

Immunogène: Synthetic peptide DDYRYRDNYEGYAPND (aa 2-17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Clone: 246E8

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1 : 1000 (AP staining)
ICC: 1 : 100 up to 1 : 500
IHC: 1 : 200
IHC-P: 1 : 500

Commentaires:

WB: SV2 aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide (= 0.02 µg)

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Stock: -20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Références pour anticorps anti-SV2B (ABIN1742257)

Aroeira, Vaz, Sebastião, Valente: "BDNF modulates glycine uptake in hippocampal synaptosomes by decreasing membrane insertion of glycine transporter 2." dans: Neurochemistry international, Vol. 99, pp. 94-102, (2016) (PubMed).

Dagar, Nagar, Goel, Cherukuri, Dhingra: "Loss of photoreceptors results in upregulation of synaptic proteins in bipolar cells and amacrine cells." dans: PLoS ONE, Vol. 9, Issue 3, pp. e90250, (2014) (PubMed).

Détails sur SV2B

Antigène: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Autre désignation: SV2 B (SV2B Produits)

Synonymes: anticorps SV2B, anticorps A830038F04Rik, anticorps AI850768, anticorps mKIAA0735, anticorps HsT19680, anticorps synaptic vesicle glycoprotein 2B, anticorps synaptic vesicle glycoprotein 2 b, anticorps synaptic vesicle glycoprotein 2b, anticorps SV2B, anticorps CpipJ_CPIJ009611, anticorps Sv2b