NSF anticorps (AA 733-744)

N° du produit ABIN1742270

Détail du produit anti-NSF anticorps

Antigène: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Épitope: AA 733-744

Reactivité: Humain, Rat, Souris, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NSF est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificité: Specific for NSF.

Purification: antiserum

Immunogène: Synthetic peptide REEGASPLDFD (aa 733-744 in rat NSF) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Information d'application

Indications d'application: WB: 1 : 1000 (AP staining)
ICC: 1 : 100 up to 1 : 1000
IHC: 1 : 200

Commentaires:

ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742269 as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS

Conseil sur la manipulation: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Stock: 4 °C/-20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Références pour anticorps anti-NSF (ABIN1742270)

de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." dans: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Tian, Wu, Unzicker, Lu, Cai, Li, Schirra, Matti, Stevens, Deng, Rettig, Sheng: "The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 45, pp. 10546-55, (2005) (PubMed).

Tailleux, Neyrolles, Honoré-Bouakline, Perret, Sanchez, Abastado, Lagrange, Gluckman, Rosenzwajg, Herrmann: "Constrained intracellular survival of Mycobacterium tuberculosis in human dendritic cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 1939-48, (2003) (PubMed).

Michaut, Tomes, De Blas, Yunes, Mayorga: "Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 18, pp. 9996-10001, (2000) (PubMed).

Détails sur NSF

Antigène: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Autre désignation: NSF (NSF Produits)

Synonymes: anticorps SKD2, anticorps AI316878, anticorps AU020090, anticorps AU067812, anticorps NSF, anticorps 18.m06598, anticorps nsf, anticorps si:bz18k17.1, anticorps wu:fj33g11, anticorps N-ethylmaleimide sensitive factor, anticorps T1J1.4, anticorps T1J1_4, anticorps NEM-sensitive fusion protein, anticorps N-ethylmaleimide sensitive factor, vesicle fusing ATPase, anticorps N-ethylmaleimide sensitive fusion protein, anticorps N-ethylmaleimide-sensitive factor, anticorps N-ethylmaleimide sensitive factor, anticorps N-ethylmaleimide-sensitive fusion protein, putative, anticorps N-ethylmaleimide sensitive factor L homeolog, anticorps vesicle-fusing ATPase, anticorps N-ethylmaleimide-sensitive factor a, anticorps AAA-type ATPase family protein, anticorps NSF, anticorps Nsf, anticorps nsf, anticorps BBOV_II007200, anticorps TGME49_318510, anticorps nsf.L, anticorps LOC100180685, anticorps LOC9310886, anticorps nsfa