UNC13A/Munc13-1 anticorps (AA 3-317)

N° du produit ABIN1742276

L’anticorps Souris Monoclonal anti-UNC13A/Munc13-1 a été validé pour WB, ELISA et IP. Il convient pour détecter UNC13A/Munc13-1 dans des échantillons de Souris, Rat et Poisson zèbre (Danio rerio). Il y a 6+ publications disponibles.

Aperçu rapide pour UNC13A/Munc13-1 anticorps (AA 3-317) (ABIN1742276)

Antigène: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Reactivité: Souris, Rat, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp UNC13A/Munc13-1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 266B1

Détail du produit anti-UNC13A/Munc13-1 anticorps

Épitope: AA 3-317

Specificité: Specific for munc 13-1.

Immunogène: Recombinant rat munc 13-1 (aa 3-317).

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1:500 up to 1:5000,

Commentaires:

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: limited to methanol fixation. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100µl H2O to get a 1mg/ml solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Agent conservateur: Sodium azide

Conseil sur la manipulation: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Stock: -20 °C

Stockage commentaire: Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.

Références pour anticorps anti-UNC13A/Munc13-1 (ABIN1742276)

Kaeser-Woo, Younts, Yang, Zhou, Wu, Castillo, Südhof: "Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 23, pp. 9769-80, (2013) (PubMed).

Dudenhöffer-Pfeifer, Schirra, Pattu, Halimani, Maier-Peuschel, Marshall, Matti, Becherer, Dirks, Jung, Lipp, Hoth, Sester, Krause, Rettig: "Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes." dans: Traffic (Copenhagen, Denmark), Vol. 14, Issue 7, pp. 798-809, (2013) (PubMed).

Maas, Torres, Altrock, Leal-Ortiz, Wagh, Terry-Lorenzo, Fejtova, Gundelfinger, Ziv, Garner: "Formation of Golgi-derived active zone precursor vesicles." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 32, pp. 11095-108, (2012) (PubMed).

Martín, Bartolomé-Martín, Torres, Sánchez-Prieto: "Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals." dans: Journal of neurochemistry, Vol. 116, Issue 4, pp. 476-85, (2011) (PubMed).

Martín, Durroux, Ciruela, Torres, Pin, Sánchez-Prieto: "The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals." dans: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17907-17, (2010) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." dans: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Détails sur UNC13A/Munc13-1

Antigène: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Autre désignation: Munc 13-1

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis